in frame mutation Search Results


human dmd patient fibroblast cells harboring out-of-frame deletion mutations of dystrophin exons 46–50  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research human dmd patient fibroblast cells harboring out-of-frame deletion mutations of dystrophin exons 46–50
(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and <t>DMD</t> <t>patient</t> <t>fibroblast</t> cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.
Human Dmd Patient Fibroblast Cells Harboring Out Of Frame Deletion Mutations Of Dystrophin Exons 46–50, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+frame+mutation/human+dmd+patient+fibroblast+cells+harboring+out+of+frame+deletion+mutations+of+dystrophin+exons+46+50/pmc05957359-46-0-35
Average 90 stars, based on 1 article reviews
human dmd patient fibroblast cells harboring out-of-frame deletion mutations of dystrophin exons 46–50 - by Bioz Stars, 2026-08
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mutation, in-frame deletion of phenylalanine 508 (f508del)  (PopulationGenetics)
90
PopulationGenetics mutation, in-frame deletion of phenylalanine 508 (f508del)
(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and <t>DMD</t> <t>patient</t> <t>fibroblast</t> cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.
Mutation, In Frame Deletion Of Phenylalanine 508 (F508del), supplied by PopulationGenetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+frame+mutation/mutation++in+frame+deletion+of+phenylalanine+508++f508del+/pmc02998727-35-9-3
Average 90 stars, based on 1 article reviews
mutation, in-frame deletion of phenylalanine 508 (f508del) - by Bioz Stars, 2026-08
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frame shift mutation  (Bellus Health)
90
Bellus Health frame shift mutation
(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and <t>DMD</t> <t>patient</t> <t>fibroblast</t> cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.
Frame Shift Mutation, supplied by Bellus Health, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+frame+mutation/frame+shift+mutation/pm30286481-285-13-7
Average 90 stars, based on 1 article reviews
frame shift mutation - by Bioz Stars, 2026-08
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nonpolar mutations in each open reading frame of the gum cluster  (Synergen Inc)
90
Synergen Inc nonpolar mutations in each open reading frame of the gum cluster
(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and <t>DMD</t> <t>patient</t> <t>fibroblast</t> cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.
Nonpolar Mutations In Each Open Reading Frame Of The Gum Cluster, supplied by Synergen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+frame+mutation/nonpolar+mutations+in+each+open+reading+frame+of+the+gum+cluster/pm01444258-220-14-1
Average 90 stars, based on 1 article reviews
nonpolar mutations in each open reading frame of the gum cluster - by Bioz Stars, 2026-08
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frame shift mutation in the foxc2 gene  (5 PRIME)
90
5 PRIME frame shift mutation in the foxc2 gene
(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and <t>DMD</t> <t>patient</t> <t>fibroblast</t> cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.
Frame Shift Mutation In The Foxc2 Gene, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+frame+mutation/frame+shift+mutation+in+the+foxc2+gene/pm28223138-205-10-21
Average 90 stars, based on 1 article reviews
frame shift mutation in the foxc2 gene - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


(A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and DMD patient fibroblast cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.

Journal: PLoS ONE

Article Title: Antisense PMO cocktails effectively skip dystrophin exons 45-55 in myotubes transdifferentiated from DMD patient fibroblasts

doi: 10.1371/journal.pone.0197084

Figure Lengend Snippet: (A) Schematic diagram of MYOD1 expression vector. (B) Histogram comparison of ZsGreen fluorescence signal vs cell number between healthy and DMD patient fibroblast cells transduced with MYOD1 expression vector. Representative images shown, although results between either patient cell line were similar. (C) Immunocytochemistry of transduced fibroblasts following 18 d (MYOD1), 15 d (MyHC, myosin heavy chain), 18 d (desmin), and 24 d (dystrophin) differentiation, respectively. Pictured are results from a healthy cell line. Nuclei counterstained with DAPI. Scale bars: 100 μm. (D) RT-PCR time-course analysis of dystrophin expression in healthy and patient DMD transdifferentiated fibroblasts. Images are representative, with dystrophin expression observed in all transdifferentiated cell lines.

Article Snippet: Human DMD patient fibroblast cells harboring out-of-frame deletion mutations of dystrophin exons 45–50 (ID: GM05017) and exons 46–50 (ID: GM05162), as well as healthy human fibroblasts (ID: GM23815) were originally obtained in 2012 from the Coriell Institute for Medical Research (Camden, NJ, USA).

Techniques: Expressing, Plasmid Preparation, Comparison, Fluorescence, Transduction, Immunocytochemistry, Reverse Transcription Polymerase Chain Reaction

(A) RT-PCR for dystrophin following cocktail PMO transfection in transdifferentiated DMD patient cells. Cells were treated with 1, 3, or 10 μM each PMO. Expected molecular weight of dystrophin exons 45–55 skipped mRNA is 308 bp. (B) Representative immunocytochemistry of transduced DMD fibroblasts following PMO cocktail transfection. Nuclei counterstained with DAPI. Scale bars: 100 μm.

Journal: PLoS ONE

Article Title: Antisense PMO cocktails effectively skip dystrophin exons 45-55 in myotubes transdifferentiated from DMD patient fibroblasts

doi: 10.1371/journal.pone.0197084

Figure Lengend Snippet: (A) RT-PCR for dystrophin following cocktail PMO transfection in transdifferentiated DMD patient cells. Cells were treated with 1, 3, or 10 μM each PMO. Expected molecular weight of dystrophin exons 45–55 skipped mRNA is 308 bp. (B) Representative immunocytochemistry of transduced DMD fibroblasts following PMO cocktail transfection. Nuclei counterstained with DAPI. Scale bars: 100 μm.

Article Snippet: Human DMD patient fibroblast cells harboring out-of-frame deletion mutations of dystrophin exons 45–50 (ID: GM05017) and exons 46–50 (ID: GM05162), as well as healthy human fibroblasts (ID: GM23815) were originally obtained in 2012 from the Coriell Institute for Medical Research (Camden, NJ, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Molecular Weight, Immunocytochemistry